Antioxidant Activity and Lipid Peroxidation Status During the Period of Hibernation in Indian Pond Frog, Euphlytes hexadactyla Lesson, 1834

Suman Pratihar, Surajit Sen, Tanmay Bhattacharya


The rate of lipid peroxidation and activities of catalase and reduced glutathione were analyzed in the brain and liver tissue of Euphlytes hexadactyla upon pre-hibernation (28.4 ± 3°C), in initial hibernation (17.2 ± 2°C) and deep hibernation (10.0 ± 2°C). During late hibernation lipid peroxidation decreased significantly as compared to pre-hibernating individuals. Increased antioxidant activity is observed in both tissues of late hibernating individuals whereas reduced glutathione activity remains at the level of pre-hibernating animals in brain tissue. Such changes appear to be physiological adaptations to combat cold stress during hibernation.


Euphlytes hexadactyla; hibernation; glutathione and catalase activities; lipid peroxidation

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