Antioxidant Activity and Lipid Peroxidation Status During the Period of Hibernation in Indian Pond Frog, Euphlytes hexadactyla Lesson, 1834

Suman Pratihar, Surajit Sen, Tanmay Bhattacharya

Abstract


The rate of lipid peroxidation and activities of catalase and reduced glutathione were analyzed in the brain and liver tissue of Euphlytes hexadactyla upon pre-hibernation (28.4 ± 3°C), in initial hibernation (17.2 ± 2°C) and deep hibernation (10.0 ± 2°C). During late hibernation lipid peroxidation decreased significantly as compared to pre-hibernating individuals. Increased antioxidant activity is observed in both tissues of late hibernating individuals whereas reduced glutathione activity remains at the level of pre-hibernating animals in brain tissue. Such changes appear to be physiological adaptations to combat cold stress during hibernation.

Keywords


Euphlytes hexadactyla; hibernation; glutathione and catalase activities; lipid peroxidation

Full Text:

PDF

References


Aebi H. (1984), «Catalase in vitro», Meth. Enzymol., 105, 121 – 126.

Babnyukova T. V. and Storey K. B. (2003), «Induction of oxidative stress in Rana ridibunda during recovery from winter hibernation», J. Thermal Biol., 28, 21 – 28.

Carey H. V. (2000), Life in Cold. Int. Hibernation Symp.

Carey H. V., Rhoads C. A., and Aw T. Y. (2003), «Hibernation induces glutathione redox imbalance in ground squirrel intestine», Comp. Biol. Physiol., 173, 269 – 276.

Emirbekof E. Z., L’vova S. P., and Gasangadzhieva A. G. (1998), «Effect of repeated cold stress on the rate of lipid peroxidation and tissue antioxidant system», Bull. Exp. Biol. Med., 125(5), 385 – 387.

Frank C. L. and Dierenfeld E. S. (1998), «The relationship between lipid peroxidation, hibernation and food selection in mammals», Am. Zoologist, 38, 341 – 349.

Griffith O. W. (1980), «Determination of glutathione and glutathione sulfide using glutathione reductase and 2-vinyl pyridine», Annu. Rev. Biochem., 106, 207.

L’vova S. P. and Gasangadzhieva A. G. (2003), «Lipid peroxidation and status of the antioxidant system in tissue of little suslik in course of hibernation», Biol. Bull., 2003, 658 – 661.

L’vova S. P., Gorbunova T. F., and Abava E. M. (1993), «The effect of hypothermia and dalargin on lipid peroxidation in rat tissue», Vopr. Med. Khimii, 39, 21 – 24 [in Russian].

Ohta H., Okamoto I., Hanaya T., and Arai S. (2006), «Enhanced antioxidant defense due to extracellular catalase activity in Syrian hamster during arousal from hibernation», Comp. Biol. Physiol., 143, 484 – 491.

Osborne P. G. and Hasimoto M. (2006), «Brain antioxidant levels in hamsters, arousal and cenothermia», Behav. Brain Tissue, 168, 208 – 214.

Page M. M. and Peters C. W. (2009), «Intracellular antioxidant enzymes are not globally upregulated during hibernation in major oxidative stress tissues of 13 lined ground squirrel, Spermarhilus tridecemlineatus», Comp. Biol. Physiol., 152, 115 – 122.

Storey K. B. (2004), «Functional metabolism», in: Regulation and Adaptation, Wiley – IEEE, USA, pp. 450 – 452.

Zenkov N. K. and Men’shchikova E. B. (1993), «Activated oxygen metabolites in biological systems», Usp. Sovr. Biol., 113, 286 – 296 [in Russian].




DOI: http://dx.doi.org/10.30906/1026-2296-2019-17-%25s-101-104

Refbacks

  • There are currently no refbacks.