Heterochromosomes and Nor-Localization in the Common Lizard, Lacerta vivipara Jacquin, from Russia

Valentina F. Orlova, A. I. Kozlovskii

Abstract


Karyotypes of the common lizard, Lacerta vivipara (2n = 36 in males; 2n = 35 in females) from Moscow, Tver’, and Ivanovo regions were studied using standard and differentiated staining. For the first time sex chromosomes of females were reliably identified with the help of G-staining: subtelocentric W and acrocentric Z1, Z2. Successive staining (G-NOR) demonstrated localization of the ribosomal gene clusters in the telomer zones of the 15th autosome pair. Analysis of the metaphase plates series stained by silver allowed the determination of the frequency of NOR activity in one or both homologues as well as the frequency of associations between them.

Keywords


Reptilia; Sauria; Lacertidae; Lacerta vivipara; chromosomes; differentiated staining; Russia

Full Text:

PDF

References


Belcheva R. G., Biserkov V. Y., Ilieva H. L., Beschkov V. A., and Petkov P. M. (1986), «Kariological studies on Lacerta vivipara (Jacq.) collected in Bulgaria», Tsitologiya [in Russian], 51(3), 567 – 570.

Chevalier M. (1969), «Données nouvelles sur le caryotipe du Lézard vivipare (Reptile, Lacertilien). Existe-t-il, une heterogametic femelle de type Z1Z2W?», C. R. Acad. Sci., 268(16), 2098 – 2100.

Chevalier M., Dufaure J. P., and Lecher P. (1979), «Cytogenetic study of Lacerta (Lacertidae, Reptilia) with particular reference to sex chromosomes», Genetica, 50(1), 11 – 18.

Howell W. M. and Black D. A. (1980), «Controlled silver staining of nucleolus organizer regions with a protective colloidal developer in a one step method», Experientia, 36(8), 1014 – 1015.

Kupriyanova L. A. (1986), «Possible pathways of karyotype evolution in lizards», in: Proc. of the Zool. Inst. Issue 157. Systematics and Ecology of Amphibians and Reptiles [in Russian], pp. 86 – 100.

Kupriyanova L. A. (1995), «New data on the distribution of Lacerta vivipara in the Europaean part of the former Soviet Union (FSU) and in western Europe: chromosomal analysis», in: Abstrs. of the 8th Ord. General Meeting of Soc. Eur. Herpetol., August 23 – 27, 1995, Bonn, p. 75.

Kupriyanova L. A. and Rudi E. R. (1990), «Comparative karyological analysis of Lacerta vivipara (Lacertidae, Sauria) populations», Zool. Zh. [in Russian], 69(6), 93 – 101.

Odierna G., Capriglione T., Caputo V., and Olmo E. (1993a), «Chromosome G-banding comparison among some Mediterranean lacertid lizards», in: Lacertids of the Mediterranean Region, E. D. Valakos, W. Bohme, V. Pérez-Mellado, and P. Maragou (eds.), Athens – Bonn – Alicante, pp. 51 – 59.

Odierna G., Kupriyanova L. A., Capriglione T., and Olmo E. (1993b), «Further data on sex chromosomes of Lacertidae and a hypothesis on their evolutionary trend», Amphibia – Reptilia, 14, 1 – 11.

Odierna G., Olmo E., and Cobror O. (1987), «Taxonomic implications of NOR-localization in lacertid lizards», Amphibia – Reptilia, 8, 373 – 382.

Olmo E., Odierna G., and Capriglione T. (1987), «Evolution of sex chromosomes in lacertid lizards», Chromosoma, 96, 33 – 38.

Ohno S., Trujillo J. M., Kaplan W. D., and Kinosita R. (1961), «Nucleolus organizers in the causation of chromosomal anomalies in man», Lancet, 2, 123 – 126.

Orlova V. F. and Kozlovskii A. I. (1995) «Identification of heterochromosomes and NOR-localization in common lizard, Lacerta vivipara Jacquin from Russia», in: Abstrs. of the 8th Ord. General Meeting of Soc. Eur. Herpetol., August 23 – 27, 1995, Bonn, p. 91.

Orlova V. F. and Orlov V. N. (1969), «Chromosome complements and some questions of systematics of lizards of the genus Lacerta», Zool. Zh. [in Russian], 48(7), 1056 – 1060.

Prokof’eva-Bel’govskaya A. A. (1966), «The nature of human acrocentric chromosome associations», Tsitologiya [in Russian], 8(2), 169 – 178.

Samner A. T., Evans H. J., and Buckland R. A. (1971), «A new technique for distinguishing between human chromosomes», Nature New Biol., 232, 31 – 32.


Refbacks

  • There are currently no refbacks.