Comparative Study of DNA Repetitive Sequences by Means of Restriction Endonucleases Among Populations and Subspecies of Some Lacertid Lizard Species

D. M. Ryabinin, V. V. Grechko, Ilya S. Darevsky, A. P. Ryskov, S. K. Semenova


As was shown earlier with reference to several animal taxa, characterization of highly repetitive DNA sequences by their susceptibility to restriction endonucleases (DNA taxoprinting) does not exhibit any differences between species populations. This method was used in our study on the populations and subspecies of some lacertid lizards: Lacerta raddei, L. saxicola, L. rudis, and L. agilis. These features were shown to be practically indistinguishable in two Armenian populations (Gosh and Khosrov) of L. raddei; the third population (Egegnadzor) demonstrates some differences. Two subspecies of Caucasian L. rudis and five subspecies of L. agilis inhabiting distant parts of the extensive range have minimal dissimilarities in the taxoprints studied. Comparison of L. saxicola subspecies, three of which inhabit the Caucasus and one the Crimea (L. s. lindholmi) presented no significant differences in any of the taxoprints of Caucasian subspecies studied up to now, while Crimean subspecies is significantly dissimilar, not less than other species are dissimilar from each other. It was suggested that L. s. lindholmi may be considered as an individual species. The molecular characteristics of genomic DNA used seem to be useful in animal taxonomy.


Lacertidae; lizards; systematics; highly repetitive DNA phylogeny; molecular phylogeny

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Bannikova A. A., Fedorova L. V., Fedorov A. N., et al. (1995), «A comparison of repetitive DNA sequences of the family Erinaceidae (Mammalia) by restriction endonuclease analysis», Genetika [in Russian], 31, 1498 – 1506.

Bobyn M. L., Darevsky I. S., Kupriyanova L. A., MacCulloch R. D., Upton D. E., Danielyan F. D., and Murphy R. W. (1996), «Allozyme variation in populations of Lacerta raddei and Lacerta nairensis (Sauria: Lacertidae) from Armenia», Amphibia – Reptilia, 17, 233 – 246.

Chelomina G. N., Lyapunova E. A., Vorontsov N. N., and Suchiya K. (1995), «Molecular-genetic typing of the three specimens of Transpaleoarctic genus of forest and field mice (Apodemus, Muridae, Rodentia)», Genetika [in Russian], 31, 820 – 824.

Christie N. T. and Skinner D. M. (1979), «Evidence for nonrandom alteration in the fraction of the highly repetitive DNA of eukaryote», Nucleic Acids Res., 6, 781 – 796.

Cooke H. J. (1975), «Evolution of the long range structure of satellite DNAs in the genus Apodemus», J. Mol. Biol., 94, 87 – 99.

Darevsky I. S., Szczerbak N. N., Peters G., et al. (1976), «Systematics and intraspecies structure», in: Agile Lizard [in Russian], A. O. Yablokov (ed.), Nauka, Moscow, pp. 53 – 95.

Darevsky I. S. (1978), Rock Lizards of the Caucasus: Systematics, Ecology, and Phylogeny of Polymorphic Group of Caucasian Rock Lizards of the Subgenus Archaeolacerta, Indian National Scientific Documentation Centre, New Delhi.

Donehower L. and Gillespie D. (1979), «Restriction site periodicities in highly repetitive DNA in Primates», J. Mol. Biol., 134, 805 – 834.

Fedorov A. N., Grechko V. V., Slobodyanyuk S. Ya., Fedorova L. V., and Timokhina G. I. (1992), «Taxonomic analysis of DNA repeated sequences», Mol. Biol. [in Russian], 26, 464 – 469.

Grechko V. V., Ryabinin D. M., Fedorova L. V., Fedorov A. N., Darevsky I. S., and Ryskov A. P. (1993), «Taxonoprint analysis of DNA from some species of the family Lacertidae», Mol. Biol. [in Russian], 27, 1404 – 1414.

Grechko V. V., Fedorova L. V., Fedorov A. N., et al. (1997), «Restriction endonuclease analysis of highly repetitive DNA as a ‘taxonomic tool’», J. Mol. Evol., in press.

Grechko V. V., Ryabinin D. M., Fedorova L. V., Fedorov A. N., Ryskov A. P., and Darevsky I. S. (1997a), «The relations among Caucasian parthenogenetic and bisexual rock lizards as revealed by restriction endonucleases analysis of highly repetitive DNA (DNA taxoprinting)», Amphibia – Reptilia, in press.

Mednikov B. M., Bannikova A. A., Lomov A. A., Mel’nikova M. N., and Shubina E. A. (1995), «Restriction analysis of repetitive DNA sequences: species criteria and species origin», Mol. Biol. [in Russian], 29, 1308 – 1319.

Mel’nikova M. N., Grechko V. V., and Mednikov B. M. (1995), «Investigation of genetic divergence and polymorphisms of nuclear DNA in species and populations of domestic and wild sheep», Genetika [in Russian], 32, 955 – 965.

Peacock W. J., Dennis E. S., Elizur A., and Calaby J. N. (1981), «Repeated DNA sequences and kangaroo phylogeny», Austral. J. Biol. Sci., 34, 325 – 340.

Popov G. I. (1983), Pleistocen of the Black-Sea – Caspian Straits (Stratigrapy, Correlations, Paleofaunistics, Geological Hystory) [in Russian], Nauka, Moscow.

Potapov S. G. and Ryskov A. P. (1993), «Analysis of variability of repetitive elements of DNA of some rodent genomes at the taxonomical level», Genetika [in Russian], 29, 869 – 872.

Potapov S. G., Vassilyev V. A., Samarina O. P., and Ryskov A. P. (1994), «Molecular genetic fingerprinting of Phodopus genus representatives», Genetika [in Russian], 30, 615 – 621.

Ryskov A. P., Kudryavtsev I. V., Vassilyev V. A., Potapov S. G., Kudryavtsev P. I., and Sirko I. P. (1994), «Diagnostic potentialities of molecular-genetic approaches to the taxonomy of the tribe Bovini», Genetika [in Russian], 30, 115 – 123.

Shubina E. A. and Mednikov B. M. (1986) «The repetitive families in DNA of Far East salmon (Onchorhinchus)», Mol. Biol. [in Russian], 20, 947 – 956.

Skurikhina L. A., Oleinik A. G., Nevshchupov S. V., and Brykov V. A. (1993), «Phylogeny of Far East salmon by the data of restriction endonuclease analysis», Genetika [in Russian], 29, 1508 – 1518.

Southern E. M. (1975), «Long range periodicities in mouse satellite», J. Mol. Biol., 94, 51 – 69.

Szczerbak N. N. (1962), «Systematics of the rock lizards (Lacerta saxicola Eversmann) in the Crimea and Northern Caucasus», Zool. Zh. [in Russian], 41, 1374 – 1385.

Szczerbak N. N., Ostashko N. G., Darevsky I. S., et al. (1976), «Areas», in: Sand Lizard [in Russian], A. O. Yablokov (ed.), Nauka, Moscow, pp. 9 – 52.

Turner B. J., Edler J. F., and Laughlin T. F. (1991), «Repetitive DNA sequences and divergence of fish populations: some hopeful beginnings», J. Fish Biol., 39, 131 – 142.


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