Open Access Open Access  Restricted Access Subscription or Fee Access

A New Species of Leptobrachella (Anura: Megophryidae) from Chu Yang Sin National Park, Dak Lak Province, Southern Vietnam

Nikolay A. Poyarkov, Tan Van Nguyen, Tang Van Duong, Son Xuan Le, Nikolai L. Orlov

Abstract


A new species of Asian leaf litter toad of the genus Leptobrachella is described from montane evergreen forests of Chu Yang Sin National Park, Dak Lak Province, southern Vietnam, based on an integrative analysis of molecular and morphological data. The new species, Leptobrachella rubromammata sp. nov., is assigned to the Leptobrachella applebyi species group. Morphologically, it is most similar to L. maculosa, L. kalonensis, and L. bidoupensis but can be distinguished by its conspicuous orange supra-axillary and pectoral glands and a distinctive ventral coloration pattern. The new species is genetically divergent from all congeners, with uncorrected 16S rRNA gene distances ranging from 6.56% to 14.55%. Based on its restricted distribution and potential vulnerability to habitat disturbance, we propose that Leptobrachella rubromammata sp. nov. be listed as Vulnerable (VU) under the IUCN Red List Categories and Criteria.

Keywords


Leptolalax applebyi species group; molecular analyses; morphology; new species; taxonomy; Lang Bian Plateau

Full Text:

PDF

References


Bragin M. A., Geissler P., Trofimets A. V., Neang T., Le S. X., Nguyen T. V., and Poyarkov N. A. (2025), «A new species of skink from mountains of southern Vietnam (Reptilia, Squamata, Scincidae)», Curr. Stud. Herpetol., 25(1 – 2), 3 – 21.

Brakels P., Maury N., and Nguyen T. V. (2021), «Geographic distribution: Leptobrachella firthi», Herpetol. Rev., 52(4), 792.

Chen J. M., Poyarkov, N. A., Suwannapoom C., Lathrop A., Wu Y. H., Zhou W. W., Zhou W. W., Yuan Z. Y., Jin J. Q., Chen H. M., Liu H. Q., Nguyen T. Q., Nguyen S. N., Duong T. V., Eto K., Nishikawa K., Matsui M., Orlov N. L., Stuart B. L., Brown R. M., Rowley J. J. L., Murphy R. W., Wang Y. Y., and Che J. (2018), «Large-scale phylogenetic analyses provide insights into unrecognized diversity and historical biogeography of Asian leaflitter frogs, genus Leptolalax (Anura, Megophryidae)», Mol. Phylogen. Evol., 124, 162 – 171.

Chen J. M., Xu K., Poyarkov N. A., Wang K., Yuan Z. Y., Hou M., Suwannapoom C., Wang J., and Che J. (2020), «How little is known about ‘the little brown frogs’: description of three new species of the genus Leptobrachella (Anura: Megophryidae) from Yunnan Province, China», Zool. Res., 41, 292 – 313.

Chen W. C., Peng W., Pan W., Liao N., Liu Y., and Huang Y. (2021), «A new species of Leptobrachella Smith 1925 (Anura: Megophryidae) from southern Guangxi, China», Zootaxa, 5020, 581 – 596.

Delorme M., Dubois A., Grosjean S., and Ohler A. (2006), «Une nouvelle ergotaxinomie des Megophryidae (Amphibia, Anura)», Alytes, 24(1 – 4), 6 – 21.

Dubois A. (1983), «Note préliminaire sur le genre Leptolalax Dubois, 1980 (Amphibiens, Anoures), avec diagnose d’une espèce nouvelle du Vietnam», Alytes, 2, 147 – 153.

Felsenstein J. (1985), «Confidence limits on phylogenies: an approach using the bootstrap», Evolution, 39(4), 783 – 791.

Felsenstein J. (2004), Inferring Phylogenies. Vol. 2, Sinauer Associates, Inc. Publishers, Sunderland, MA, USA.

Frost D. R. (2025), Amphibian Species of the World 6.2, an Online Reference, http: //research.amnh.org/herpetology/ amphibia/index.html (accessed July 15, 2025)

Hall T. A. (1999), «BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT», Nucleic Acids Symp. Ser., 41, 95 – 98.

Hedges S. B. (1994), «Molecular evidence for the origin of birds», Proc. Natl. Acad. Sci. USA, 91(7), 2621 – 2624.

Hillis D. M. and Bull J. J. (1993), «An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis», Syst. Biol., 42(2), 182 – 192.

Hillis D. M., Moritz C., and Mable B. K. (1996), Molecular Systematics. 2nd Edition, Sinauer Associates, Sunderland, MA, USA.

Hoang C. V., Nguyen T. T., Luu V. Q., Nguyen T. Q., and Jiang J. P. (2019), «A new species of Leptobrachella Smith, 1925 (Anura, Megophryidae) from Thanh Hoa Province, Vietnam», Raffles Bull. Zool., 67, 536 – 556.

Hoang C. V., Luong A. M., Nguyen T. T., Nguyen T. Q., Ninh H. T., Le, L. T. H., Ziegler T., and Pham C. T. (2024), «A new species of Leptobrachella Smith 1925 (Anura, Megophryidae) from Lai Chau Province, Vietnam», Biodivers. Data J., 12, e136491.

Hoang V. C., Pham T. C., Phan Q. T., Do T. D., Wang B., Jiang J. P., and Nguyen T. Q. (2025), «Hidden biodiversity in the tropical rain forests: two new species of Leptobrachella Smith 1925 (Anura: Megophryidae) from Vietnam», Asian Herpetol. Res., 16(2), 169 – 191.

Huelsenbeck J. P. and Hillis D. M. (1993), «Success of phylogenetic methods in the four-taxon case», Syst. Biol., 42, 247 – 264.

Huelsenbeck J. P. and Ronquist F. (2001), «MRBAYES: Bayesian inference of phylogeny», Bioinformatics, 17, 754 – 755.

Jestrzemski D., Schütz S., Nguyen T. Q., and Ziegler T. (2013), «A survey of amphibians and reptiles in Chu Mom Ray National Park, Vietnam, with implications for herpetofaunal conservation», Asian J. Conserv. Biol., 2(2), 88 – 110.

Jiang K., Yan F., Suwannapoom C., Chomdej S., and Che J. (2013), «A new species of the genus Leptolalax (Anura, Megophryidae) from Northern Thailand», Asian Herpetol. Res., 4(2), 100 – 108.

Jobb G., von Haeseler A., and Strimmer K. (2004), «TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics», BMC Evol. Biol., 4, 18.

Kumar S., Stecher G., and Tamura K. (2016), «MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets», Mol. Biol. Evol., 33(7), 1870 – 1874.

Lanfear R., Calcott B., Ho S. Y., and Guindon S. (2012), «Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses», Mol. Biol. Evol., 29(6), 1695 – 1701.

Lathrop A., Murphy R. W., Orlov N. L., and Ho C. T. (1998), «Two new species of Leptolalax (Anura, Megophryidae) from northern Vietnam», Amphibia–Reptilia, 19, 253 – 267.

Le T. D., Do Y. T., Tran T. T., and Nguyen T. Q. (2021), «Two new records of Leptobrachella (Anura: Megophryidae) from Vietnam», Russ. J. Herpetol., 28(4), 219 – 224.

Lo N. T., Hoang C. V., Nguyen T. Q., Nguyen S. L. H., Ziegler T., and Pham C. T. (2022), «First record of Leptobrachella shiwandashanensis Chen, Peng, Pan, Liao, Liu et Huang, 2021 (Anura: Megophryidae) from Vietnam», J. For. Sci. Technol., 14, 28 – 32.

Luong A. M., Hoang C. V., Pham C. T., Nguyen T. T., Orlov N. L., Ziegler T., and Nguyen T. Q. (2022), «A new species of Xenophrys (Amphibia: Anura: Megophryidae) from Truong Son Range, Vietnam», Zootaxa, 5150, 333 – 356.

Matsui M. (2006), «Three new species of Leptolalax from Thailand (Amphibia, Anura, Megophryidae)», Zool. Sci., 23(9), 821 – 830.

Matsui M., Panha S., and Eto K. (2023), «Two new species of Leptobrachella from northern Thailand (Amphibia, Anura, Megophryidae)», Curr. Herpetol., 42, 83 – 97.

Moran M. D. (2003), «Arguments for rejecting the sequential Bonferroni in ecological studies», Oikos, 100, 403 – 405.

Nakagawa S. (2004), «A farewell to Bonferroni: the problems of low statistical power and publication bias», Behav. Ecol., 15(6), 1044 – 1045.

Nazarov R. A., Orlov N. L., Nguyen S. N., and Ho C. T. (2008), «Taxonomy of naked-toe geckos Cyrtodactylus irregularis complex of South Vietnam and description of a new species from Chu Yang Sin Natural Park (Krong Bong District, Dac Lac Province): Vietnam», Russ. J. Herpetol., 15(2), 141 – 156.

Nguyen L. T., Poyarkov N. A., Le D. T., Vo B. D., Phan H. T., Duong T. V., Murphy R. W., and Nguyen S. N. (2018), «A new species of Leptolalax (Anura, Megophryidae) from Son Tra Peninsula, central Vietnam», Zootaxa, 4388(1), 1 – 21.

Nguyen L. T., Tapley B., Nguyen C. T., Luong H. V., and Rowley J. J. L. (2021), «A new species of Leptobrachella (Anura, Megophryidae) from Mount Pu Ta Leng, northwest Vietnam», Zootaxa, 5016, 301 – 332.

Nguyen T. V., Brakels P., Maury N., Sudavanh S., Pawangkhanant P., Idiiatullina S., Lorphengsy S., Inkhavilay K., Suwannapoom C., and Poyarkov N. A. (2020), «New herpetofaunal observations from Laos based on photo records», Amphib. Reptile Conserv., 14, 218 – 249 (e248).

Ninh H. T., Nguyen T. T., Le L. T. H., Nguyen T. V., Nguyen H. N., Orlov N. L., Bezman-Moseyko O., Le M. V., Nguyen S. N., and Ziegler T. (2024), «A new leaf-litter frog of Leptobrachella (Anura: Megophryidae) from Tay Con Linh Mountain, Northern Vietnam», Russ. J. Herpetol., 31(4), 191 – 224.

Ohler A., Marquis O., Swan S., and Grosjean S. (2000), «Amphibian biodiversity of Hoang Lien Nature Reserve (Lao Cai Province, northern Vietnam) with description of two new species», Herpetozoa, 13(1 – 2), 71 – 87.

Ohler A., Wollenberg K. C., Grosjean S., Hendrix R., Vences M., Ziegler T., and Dubois A. (2011), «Sorting out Lalos, description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura)», Zootaxa, 3147, 1 – 83.

Perneger T. V. (1998), «What’s wrong with Bonferroni adjustments», BMJ, 316(7139), 1236.

Pham A. V., Le D. T., Pham C. T., Nguyen S. H. L., Ziegler T., and Nguyen T. Q. (2016), «Two additional records of megophryid frogs, Leptobrachium masatakasatoi Matsui, 2013 and Leptolalax minimus (Taylor, 1962), for the herpetofauna of Vietnam», Rev. Suisse Zool., 123(1), 35 – 43.

Poyarkov N. A., Duong T. V., Orlov N. L., Gogoleva S. S., Vassilieva A. B., Nguyen L. T., Nguyen V. D. H., Nguyen S. N., Che J., and Mahony S. (2017), «Molecular, morphological and acoustic assessment of the genus Ophryophryne (Anura, Megophryidae) from Langbian Plateau, southern Vietnam, with description of a new species», ZooKeys, 672, 49 – 120.

Poyarkov N. A., Geissler P., Gorin V. A., Dunayev E. A., Hartmann T., and Suwannapoom C. (2019), «Counting stripes, revision of the Lipinia vittigera complex (Reptilia, Squamata, Scincidae) with description of two new species from Indochina», Zool. Res., 40(5), 358 – 393.

Poyarkov N. A., Nguyen T. V., Popov E. S., Geissler P., Pawangkhanant P., Neang T., Suwannapoom C., Ananjeva N. B., and Orlov N. L. (2023), «Recent progress in taxonomic studies, biogeographic analysis, and revised checklist of reptiles in Indochina», Russ. J. Herpetol., 30(5), 255 – 476.

Poyarkov N. A., Nguyen T. V., Popov E. S., Geissler P., Pawangkhanant P., Neang T., Suwannapoom C., and Orlov N. L. (2021), «Recent progress in taxonomic studies, biogeographic analysis and revised checklist of amphibians in Indochina», Russ. J. Herpetol., 28(3A), 1 – 110.

Poyarkov N. A., Rowley J. J. L., Gogoleva S. I., Vassilieva A. B., Galoyan E. A., and Orlov N. L. (2015), «A new species of Leptolalax (Anura, Megophryidae) from the western Langbian Plateau, southern Vietnam», Zootaxa, 3931(2), 221 – 252.

Poyarkov N. A., Vassilieva A. B., Orlov N. L., Galoyan E. A., Tran D. A. T., Le D. T. T., Kretova V. D., and Geissler P. (2014), «Taxonomy and distribution of narrow-mouth frogs of the genus Microhyla Tschudi, 1838 (Anura, Microhylidae) from Vietnam with descriptions of five new species», Russ. J. Herpetol., 21(2), 89 – 148.

Rowley J. J. L. and Cao T. T. (2009), «A new species of Leptolalax (Anura, Megophryidae) from central Vietnam», Zootaxa, 2198, 51 – 60.

Rowley J. J. L., Dau V. Q., and Cao T. T. (2017b), «A new species of Leptolalax (Anura, Megophryidae) from Vietnam», Zootaxa, 4273(1), 61 – 79.

Rowley J. J. L., Dau V. Q., and Nguyen T. T. (2013), «A new species of Leptolalax (Anura, Megophryidae) from the highest mountain in Indochina», Zootaxa, 3737(4), 415 – 428.

Rowley J. J. L., Dau V. Q., Hoang H. D., Le D. T. T., Cutajar T. P., and Nguyen T. T. (2017a), «A new species of Leptolalax (Anura, Megophryidae) from northern Vietnam», Zootaxa, 4243(3), 544 – 564.

Rowley J. J. L., Hoang H. D., Dau V. Q., Le D. T. T., and Cao T. T. (2012), «A new species of Leptolalax (Anura, Megophryidae) from central Vietnam», Zootaxa, 3321, 56 – 68.

Rowley J. J. L., Hoang H. D., Le D. T. T., Dau V. Q., and Cao T. T. (2010b), «A new species of Leptolalax (Anura, Megophryidae) from Vietnam and further information on Leptolalax tuberosus», Zootaxa, 2660, 33 – 45.

Rowley J. J. L., Le D. T. T., Tran D. A. T., and Hoang H. D. (2011a), «A new species of Leptolalax (Anura, Megophryidae) from southern Vietnam», Zootaxa, 2796, 15 – 28.

Rowley J. J. L., Stuart B. L., Neang T., and Emmett D. A. (2010a), «A new species of Leptolalax (Anura, Megophryidae) from northeastern Cambodia», Zootaxa, 2567, 57 – 78.

Rowley J. J. L., Stuart B. L., Neang T., Hoang H. D., Dau V. Q., Nguyen T. T., and Emmett D. A. (2015), «A new species of Leptolalax (Anura, Megophryidae) from Vietnam and Cambodia», Zootaxa, 4039(3), 401 – 417.

Rowley J. J. L., Stuart B. L., Richards S. J., Phimmachak S., and Sivongxay N. (2010c), «A new species of Leptolalax (Anura, Megophryidae) from Laos», Zootaxa, 2681, 35 – 46.

Rowley J. J. L., Tran D. A. T., Le D. T. T., Dau V. Q., Peloso P. L. V., Nguyen T. Q., Hoang H. D., Nguyen T. T., and Ziegler T. (2016), «Five new, microendemic Asian leaf-litter frogs (Leptolalax) from the southern Annamite mountains, Vietnam», Zootaxa, 4085(1), 63 – 102.

Rowley J. J. L., Tran D. T. A., Frankham G. J., Dekker A. H., Le D. T. T., Nguyen T. Q., Dau V. Q., and Hoang H. D. (2015), «Undiagnosed cryptic diversity in small, microendemic frogs (Leptolalax) from the central highlands of Vietnam», PLoS One, 10(5), e0128382.

Stuart B. L. and Rowley J. J. L. (2020), «A new Leptobrachella (Anura: Megophryidae) from the Cardamom Mountains of Cambodia», Zootaxa, 4834, 556 – 572.

Taylor E. H. (1962), «The amphibian fauna of Thailand», Univ. Kansas Sci. Bull., 43, 265 – 599.

Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., and Higgins D. G. (1997), «The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools», Nucleic Acids Res., 25(24), 4876 – 4882.

Vences M., Thomas M., Bonett R. M., and Vieites D. R. (2005a), «Deciphering amphibian diversity through DNA barcoding: chances and challenges», Phil. Trans. R. Soc. Lond. B. Biol. Sci., 360(1462), 1859 – 1868.

Vences M., Thomas M., Van Der Meijden A., Chiari Y., and Vieites D. R. (2005b), «Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians», Front. Zool., 2(1), 5.

Vieites D. R., Wollenberg K. C., Andreone F., Köhler J., Glaw F., and Vences M. (2009), «Vast underestimation of Madagascar’s biodiversity evidenced by an integrative amphibian inventory», Proc. Natl. Acad. Sci. USA, 106(20), 8267 – 8272.

Wu Y. H., Chen J. M., Pawangkhanant P., Yothawut C., Karuno A. P., Suwannapoom C., and Che J. (2022), «Distribution extension of Leptobrachella eos (Ohler, Wollenberg, Grosjean, Hendrix, Vences, Ziegler et Dubois, 2011): first record from Thailand», Herpetozoa, 35, 25 – 32.

Yang J., Zeng Z. C., and Wang Y. Y. (2018), «Description of two new sympatric species of the genus Leptolalax (Anura: Megophryidae) from western Yunnan of China», PeerJ, 6, e4586.




DOI: https://doi.org/10.30906/1026-2296-2025-32-4-293-308

Refbacks

  • There are currently no refbacks.



You can subscribe to the print or electronic version of the journal on the site of EastView Company. If you have any questions, please write to the email sales@ivis.ru