Antimicrobial Activity in the Skin Secretion of Hyla arborea (Linnaeus, 1758)

Basaran Dulger, Cemal Varol Tok, Serhat Kaya, Murat Sevinc


In this study, antimicrobial activity of various extracts prepared from Hyla arborea skin secretion have been investigated for their antimicrobial activity. Antimicrobial activity was determined with Escherichia coli ATCC 11230, Staphylococcus aureus 6538-P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27583, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403, and Kluyveromyces fragilis ATCC 8608 by the disk diffusion method. The extracts of all plant species had strong antimicrobial activity against both Gram-positive and Gram-negative bacteria, but no activity was seen against the yeast cultures used in this study. According to our results, the extracts prepared from Hyla arborea skin secretion have high antimicrobial activity against the tested bacteria.


Hyla arborea; Amphibia; antimicrobial activity; skin secretion

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Başoğlu M., Ozeti N., and Yilmaz I. (1994), «Amphibians of Turkey», Ege Univ. Sci. Fac. Book Ser., No. 151.

Bronmark C. and Edenhamn P. (1994), «Does the presence of fish affect the distribution of tree frogs (Hyla arborea)?» Conserv. Biol., 8(3), 841 – 845.

Cetin T. E. and Gurler N. (1989), «Bakterilerin Antibiyotiklere Duyarlilik Deneyinin Yapilmasi [The experiment for antibiotic sensitivity of bacteria]», J. Kukem, 12, 2 – 5 [in Turkish].

Cevikbas A. (1978), «Antibacterial activity in the skin secretion of the frog Rana ridibunda», Toxicon, 16(2), 195 – 197.

Collins C. M., Lyne P. M., and Grange J. M. (1989), Microbiological Methods. Sixth Edition, Butterworths, London.

Corbett K. (1989), Conservation of European Reptiles and Amphibians, Helm, Bromley (UK).

Croce R., Giglioli N., and Bolognani L. (1973), «Antimicrobial activity in the skin secretions of Bombina variegata pachymus», Toxicon, 11, 99 – 100.

Edstrom A. (1992) Venomous and Poisonous Animals, Kriger Publishing Co., Malabar (Florida).

Kolbe H. V. I., Huber A., Cordier P., Rasmussen U. B., Bouchon B., Jaquýnod M., Vlasak R., Delot E., and Kreil G. (1993), «Xenoxins, a family of peptides from dorsal gland secretion of Xenopus laevis-related to snake-venom cytotoxins and neurotoxins», J. Biol. Chem., 268(22), 16458 – 16464.

Lutz B. (1971), Venomous Animals and Their Venom, Acad. Press, London.

NCCLS (1993), Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standard. NCCLS Publication M2-A5, Villanova, PA.

Pavan M. (1962), «Die Antibiotica tierischer Herkunft», Zeit. Hyg. Infektionskr., 34, 136 – 138.

Pavan M. and Nascimbene A. (1948), «Studi sugli antibiotici di origine animale», Boll. Soc. Med. Chir. Pavia, Nos. 1 – 2, 229.

Preusser H. J., Habermehl G., Sablofski M., and Schmall H. D. (1975), «Antimicrobial activity of alkoloid from amphibian venoms and effects on the ultrastucture of yeast cells», Toxicon, 12, 285.

Sanna A. P., Barnabei A., and Delfino G. (1993), «The cutaneous venom of Bombina orientalis: cytotoxic Effects on the human HL 60 cell line and a comparison with Bombina variegata», J. Nat. Toxins, 2(2), 161 – 173.



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