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Red Blood Cell Morphology and Blood Biochemistry of Bufotes variabilis

Sevay Ayşe Ulubeli, Eyup Başkale, Yakup Kaska

Abstract


To determine blood biochemistry and reassess and compare red blood cell morphology based on individuals’ gender, age and SVL (Snout-Vent Length), we studied 30 blood samples of Bufotes variabilis (15 males, 15 females) from Ornaz Valley, Denizli/Turkey. For age determination, bone samples were threated according to the skeletochronology procedure and a strong positive correlation between SVL and age structure (Pearson’s, n = 30, r2 = 0.937, p < 0.001) was detected. Like all anurans, the shapes of the erythrocytes and their nuclei were ellipsoidal while their dimensions were bigger than expected when compared with those in the previous studies. Statistical evaluations of the red blood cells were made according to individuals’ gender, age and SVL and no significant differences were found amongst them. This is the first study about the blood biochemistry of B. variabilis which includes pH, blood gas, oximetry, electrolyte (potassium, sodium, calcium, chloride and bicarbonate ion concentrations) and metabolite [D-glucose and L(+)-lactate concentration] values. Biochemical data were statistically analysed according to gender, age and SVL to evaluate whether there are any relationships or differences between the studied individuals. Statistical analyses of the biochemical assessments showed that cCa values of females were significantly high (t-test: t = 2.30, df = 20, p < 0.05), possibly due to the spawning period. The mean value of the individuals’ age was 7.77 ± 1.33 years with the mean SVL of 80.4 ± 1.2 mm. Pearson’s correlations of the biochemical data indicated a very significant relationship between cCl ion concentration and SVL as well as age. However, highly significant positive correlations were found between sO2 – pO2, FO2Hb – pO2, FO2Hb – sO2, FHHb – pCO2, cNa – pH, cNa – cK, cCa – cK, cCa – cNa, cHO3 – pCO2 and cLac – cGlu values while pO2 – pCO2, sO2 – pCO2, FHHb – pO2, FHHb – sO2, FHHb – FO2Hb, cK – pCO2 values showed a very significant negative correlation. These are the first biochemical data and evaluations about B. variabilis blood. In addition, according to the data obtained in our study, it is possible to say that age can be used as a substitute of SVL when it is not possible to procure the exact measurements of an individual for assessment.

Keywords


Bufotes variabilis; erythrocyte; blood biochemistry; skeletochronology

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References


Arıkan H., Çevik I. E., Kaya U., and Mermer A. (2001), «Anadolu Dağ Kurbağalarında eritrosit ölçümleri [Erythrocyte measurements in Anatolian Mountain Frogs]», Anadolu Üniv. Bilim Teknol. Dergisi, 2(2), 387 – 391 [in Turkish].

Arıkan H., Alpagut-Keskin N., Çevik I. E., and Erişmiş U. C. (2010), «A study on the blood cells of the fire-bellied toad, Bombina bombina L. (Anura: Bombinatoridae)», Animal Biol., 60, 61 – 68.

Arıkan H. and Çiçek K. (2010), «Blood cell morphology of Turkish herpetofauna», Acta Herpetol., 5(2), 179 – 198.

Arıkan H. and Çiçek K. (2011), «Changes in blood-serum proteins, erythrocyte count, and size of Pelophylax bedriagae (Camerano, 1882) during metamorphosis (Anura, Ranidae)», Herpetozoa, 24, 3 – 11.

Arserim S. K. and Mermer A. (2008), «Hematology of the Uludağ frog, Rana macrocnemis Boulenger, 1885 in Uludağ National park (Bursa, Turkey)», E.U. J. Fisheries Aquatic Sci., 25(1), 39 – 46.

Atatür M. K., Arıkan H., and Mermer A. (1998), «Erythrocyte sizes of some Urodeles from Turkey», Turk. J. Zool., 22, 89 – 91.

Atatür M. K., Arıkan H., and Çevik I. E. (1999), «Erythrocyte sizes of some Anurans from Turkey», Turk. J. Zool., 23, 111 – 114.

Avci A., Tuniyev B., Isailovic J. C., Lymberakis P., Andrén C., Cogalniceanu D., Wilkinson J., Ananjeva N., Üzüm N., Orlov N., Podloucky R., Tuniyev, S. Kaya U., Nilson G., Sharifi M., Papenfuss T., and Bafti S. S. (2015), «Bufotes variabilis», in: The IUCN Red List of Threatened Species 2015, e.T153571A74506874, downloaded on 18 January 2018.

Baraquet M., Grenat P. R., Salas N. E., and Martino A. L. (2013), «Intraspecific variation in erythrocyte sizes among populations of Hypsiboas cordobae (Anura: Hylidae)», Acta Herpetol., 8, 93 – 97.

Budak A. and Göçmen B. (2008), «Clasis 1: Amphibia (= Batrachia) (Kurbağalar, İki yaşamlılar)», in: Herpetoloji. 2 Baskı [Herpetology], 2nd edition, Ege Üniv. Basımevi, Bornova-İzmir, pp. 7 – 74 [in Turkish].

Cabagna M. C., Lajmanovich R. C., Stringhini G., Sanchezhernandez J. C., and Peltzer P. M. (2005), «Hematological parameters of health status in the common toad Bufo arenarum in agroecosystems of Santa Fe Province, Argentina», Appl. Herpetol., 2, 373 – 380.

Castanet J. and Smirina E. (1990), «Introduction to the skeletochronological method in amphibians and reptiles», Ann. Sci. Nat. Zool. Paris, 11, 191 – 196.

Das M. and Mahapatra P. K. (2014), «Hematology of wild caught Dubois’s tree frog Polypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)», Sci. World J., 2014, 1 – 7.

Davis A. K. (2008), «Ontogenetic changes in erythrocyte morphology in larval mole salamanders, Ambystoma talpoideum, measured with image analysis», Comp. Clin. Pathol., 17, 23 – 28.

Davis A. K. and Durso A. M. (2009), «White blood cell differentials of northern cricket frogs (Acris c. crepitans) with a compilation of published values from other amphibians», Herpetologica, 65, 260 – 267.

Davis A. K., Milanovich J. R., DeVore J. L., and Maerz J. C. (2009), «An investigation of factors influencing erythrocyte morphology of Red-backed Salamanders (Plethodon cinereus)», Animal Biol., 59, 201 – 209.

Evans G. (1939), «Factors influencing the oxygen consumption of several species of Plethodontid Salamanders in aerial and aquatic media», Ecology, 20, 74 – 95.

Gustafson K. D., Newman R. A., Pulis E. E., and Cabarle K. C. (2015), «A skeletochronological assessment of age-parasitism relationships in Wood Frogs (Lithobates sylvaticus)», J. Herpetol., 49(1), 122 – 130.

Gül Ç., Tosunoğlu M., Erdoğan D., and Özdamar D. (2011), «Changes in the blood composition of some Anurans», Acta Herpetol., 6(2), 137 – 147.

Grenat P. R., Bionda C. L., Salas N. E., and Martino A. L. (2009), «Variation in erythrocyte size between juveniles and adults of Odontophrynus americanus», Amphibia–Reptilia, 30, 141 – 145.

Hota J., Das M., and Mahapatra P. K. (2013), «Blood cell profile of the developing tadpoles and adults of the ornate frog, Microhyla ornata (Anura: Microhylidae)», Int. J. Zool., 2013, 1 – 14.

Katz U. (1986), «The role of amphibian epidermis in osmoregulation and its adaptive response to changing environment», in: J. Bereiter-Hahn, A. G. Matoltsy, K. S. Richards (eds.), Biology of the Integument. Vol. 2. The vertebrates, Springer, Berlin – Heidelberg – New York, pp. 472 – 498.

Mahapatra B. B., Das M., Dutta S. K., and Mahapatra P. K. (2012), «Hematology of Indian rhacophorid tree frog Polypedates maculatus Gray, 1833 (Anura: Rhacophoridae)», Comp. Clin. Pathol., 21(4), 453 – 460.

Peavy T. R., Hernandez C., and Carroll E. J., Jr. (2003), «Jeltraxin, a frog egg jelly glycoprotein, has calcium-dependent lectin properties and is related to human serum pentraxins CRP and SAP», Biochemistry, 42, 12761 – 12769.

Smirina E. M. (1994), «Age determination and longevity in amphibians», Gerontology, 40, 133 – 146.

Van Aardt W. J. and Weber R. E. (2010), «Respiration and hemoglobin function in the giant African bullfrog Pyxicephalus adspersus Tschudi (Anura: Pyxicephalidae) during rest, exercise and dormancy», Afr. J. Herpetol., 59(2), 173 – 190.

Vernberg F. J. (1955), «Correlation of physiological and behaviour indexes of activity in the study of Plethodon glutinosus (Green)», Am. Midland Naturalist, 54, 382 – 393.

Vitt L. J. and Caldwell J. P. (2014), Herpetology. An Introductory Biology of Amphibians and Reptiles. 4th Edition, Acad. Press, San Diego.

Welsh H. H. and Ollivier L. M. (1998), «Stream amphibians as indicators of ecosystem stress: a case study from California’s redwoods», Ecol. Appl., 8(4), 1118 – 1132.

Wojtaszek J. and Adamowicz A. (2003), «Haematology of the fire-bellied toad, Bombina bombina L. », Comp. Clin. Pathol., 12, 129 – 134.

Young S., Warner J., Speare R., Berger L., Skerratt L. F., and Muller R. (2012), «Hematologic and plasma biochemical reference intervals for health monitoring of wild Australian tree frogs», Vet. Clin. Pathol., 41(4), 478 – 492.




DOI: https://doi.org/10.30906/1026-2296-2020-27-6-316-324

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