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Urinal Sperm Motility and Parasite Load in Anuran Amphibians (Anura)

Аnna Vladimirovna Burakova, Ekaterina Aleksandrovna Baitimirova, Ivan Aleksandrovich Kshnyasev, Ilya Vladimirovich Zubarev


Parasite load effect on the spermatozoa motility of the moor frog (Rana arvalis Nilsson, 1842) native urinal sperm has been evaluated for the first time. Seven species of parasites were identified: Nematoda — Oswaldocruzia filiformis (Goeze, 1782), Neoraillietnema praeputiale (Skrjabin, 1916), Cosmocerca ornata (Dujardin, 1845), Rhabdias bufonis (Schrank, 1788); Trematoda — Dolichosaccus rastellus (Olsson, 1876), Haplometra cylindracea (Zeder, 1800); Opalinatea — Opalina ranarum (Dujardin, 1841). We found no impact of population specificity on sperm motility. The strong negative correlation between motile spermatozoa proportion and O. ranarum abundance was observed. For the purpose of selecting the optimal model of dependence pattern (linear-, logarithmic-, sigmoid- or threshold function), the Consistent Akaike Information Criterion (CAIC) was used. When the O. ranarum abundance was above the estimated threshold — 7.3 (95% CI 0.9 – 13.6) trophonts per probe, the expected probability to observe motile spermatozoa in the urinal sperm of moor frog decreased by 3.6 (95% CI for Risk Ratio: 1.9 – 6.8) times.


Rana arvalis; parasites; protozoa; Opalina ranarum; urinal sperm; sperm motility; parasite load

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