Isolation and Development of Highly Polymorphic Microsatellites in Rhacophorus hui

Feng-Hui Sun, Yi-Tao Zheng, Jing Wang, Sebastian Klaus, Hai-Bin Xie, Jia-Tang Li


Rhacophorus hui and Rhacophorus dugritei were once considered as parts of the same species for their morphological similarity, and subsequent studies found the chromosomal inversions in their genome made them separated into two different species finally. To explore the relationship between the two species and perform population genetics analysis of R. hui, highly polymorphic microsatellite markers were required. Here we reported the isolation and characterization of thirteen highly polymorphic novel microsatellite markers of R. hui for phylogenetic and population genetic analysis. The number of alleles per locus ranged from 12 to 29, and the average heterozygosity observed and expected per locus ranged from 0.429 to 0.930 and 0.848 to 0.959, respectively. As for sequential Bonferroni corrections, three pairs showed significant departure from Hardy-Weinberg equilibrium. These polymorphic loci will be useful markers in the research of R. hui.


microsatellite markers; Rhacophorus hui; Rhacophorus dugritei; Rhacophorus dugritei complex; highly polymorphic loci; paracentric inversion

Full Text:



Benson G. (1999), «Tandem Repeats Finder: A program to analyze DNA sequences», Nucl. Acids Res., 27, 573 – 580.

David A. (1872), «Rapport adressé a MM. les Professeurs-Administráteurs du Museum d’histoire naturelle», Nouv. Arch. Mus., 7, 75 – 100.

Fei L., Ye C. Y., and Jiang J. P. (2010), Colored Atlas of Chinese Amphibians, Sichuan Publishing House of Science and Technology press, Chengdu, China

Guivhoux E. and Lagache L. (2011), «Current trends in microsatellite genotyping», Mol. Ecol. Resour., 11, 591 – 611.

Landis J. B., Hudman S. P., Grose M. J., Skalski G. T., and Wiley E. O. (2009), «Characterization of 32 novel microsatellite loci for population and mating system studies using Campostoma anomalum (central stoneroller)», Mol. Ecol. Resour., 9, 251 – 254.

Li J. T., Wu G. F., and Zhao E. M. (2006), «A karyotypic study on Polypedates dugritei and P. zhaojuensis with discussion on their taxonomic status», Sichuan J. Zool., 25, 12 – 16.

Li J. T., Liu J., Chen Y. Y., Wu J. W., Murphy R. W., Zhao E. M., Wang Y. Z., and Zhang Y. P. (2012), «Molecular phylogeny of treefrogs in the Rhacophorus dugritei species complex (Anura: Rhacophoridae), with descriptions of two new species», Zool. J. Linn. Soc. Lond., 5, 143 – 162.

Liu C. C. (1945), «New frogs from west China», J. West China Border Res. Soc., 15, 28 – 43.

Liu C. C. and Hu S. Q. (1961), Chinese Tailless Amphibians, Science Press, Beijing.

Raymond M. and Rousset F. (1995), «Genepop ver. 1.2: population genetics software for exact tests and ecumenicism», J. Hered., 86, 248 – 249.

Rice W. R. (1989), «Analysing tables of statistical tests», Evolution, 43, 223 – 225.

Rousset F. (2008), «A complete re-implementation of the GenePop software for Windows and Linux», Mol. Ecol. Resour., 8, 103 – 106.

Sunnucks P. and Hales D. F. (1996), «Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae)», Mol. Biol. Evol., 13, 510 – 524.

Wu G. F. and Zeng X. M. (1994), «The karyotypic differentiation of Polypedates dugritei with description of a superspecies (Rhacophoridae, Anura)», Sichuan J. Zool., 4, 156 – 161.

Zane L., Bargelloni L., and Patarnello T. (2002), «Strategies for microsatellite isolation: a review», Mol. Ecol., 11, 1 – 16.



  • There are currently no refbacks.

You can subscribe to the print or electronic version of the journal on the site of EastView Company. If you have any questions, please write to the email